DNA barcode trnH-psbA is a promising candidate for efficient identification of forage legumes and grasses
نویسندگان
چکیده
منابع مشابه
Species identification of medicinal pteridophytes by a DNA barcode marker, the chloroplast psbA-trnH intergenic region.
Medicinal pteridophytes are an important group used in traditional Chinese medicine; however, there is no simple and universal way to differentiate various species of this group by morphological traits. A novel technology termed "DNA barcoding" could discriminate species by a standard DNA sequence with universal primers and sufficient variation. To determine whether DNA barcoding would be effec...
متن کاملIntraspecific Inversions Pose a Challenge for the trnH-psbA Plant DNA Barcode
BACKGROUND The chloroplast trnH-psbA spacer region has been proposed as a prime candidate for use in DNA barcoding of plants because of its high substitution rate. However, frequent inversions associated with palindromic sequences within this region have been found in multiple lineages of Angiosperms and may complicate its use as a barcode, especially if they occur within species. METHODOLOGY...
متن کاملEvaluation of ITS2 for intraspecific identification of Paeonia lactiflora cultivars
Herbaceous peony (Paeonia lactiflora Pall.) is an important ornamental and medicinal plant. DNA barcodes can reveal species identity via the nucleotide diversity of short DNA segments. In this study, two main candidate DNA barcodes (ITS2 and psbA-trnH) were tested to identify twenty-one cutting cultivars of P. lactiflora and their wild species. The efficacy of the candidate DNA barcodes was ass...
متن کاملEvaluation of six candidate DNA barcode loci for identification of five important invasive grasses in eastern Australia
Invasive grass weeds reduce farm productivity, threaten biodiversity, and increase weed control costs. Identification of invasive grasses from native grasses has generally relied on the morphological examination of grass floral material. DNA barcoding may provide an alternative means to identify co-occurring native and invasive grasses, particularly during early growth stages when floral charac...
متن کاملMolecular identification of Uncaria (Gouteng) through DNA barcoding
BACKGROUND While DNA barcoding is an important technology for the authentication of the botanical origins of Chinese medicines, the suitable markers for DNA barcoding of the genus Uncaria have not been reported yet. This study aims to determine suitable markers for DNA barcoding of the genus Uncaria (Gouteng). METHODS Genomic DNA was extracted from the freshly dried leaves of Uncaria plants b...
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ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2020
ISSN: 1756-0500
DOI: 10.1186/s13104-020-4897-5